Drosophila food medium
(3l)
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Ingredients
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27 g agar
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Dissolve agar in 1 l tap water by boiling
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200 g cornmeal
140 g sugar
50 g dry yeast
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In the meanwhile, dissolve cornmeal, sugar and dry
yeast in 2 l of cold tap water. When the agar-solution is boiling,
add the corn-sugar-yeast-solution by and by. Stir constantly to
prevent burning.
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10 ml propionic acid
a dash Nipagin
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After 5 minutes of gentle boiling, reduce the heat
and add the propionic acid and the Nipagin to prevent the medium
from moldering.
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dissolved yeast cake
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Fill the hot medium into vials and let it chill
down to room temerature. When the medium is lukewarm, place a droplet
of a thick suspension of fresh yeast cake in water on the medium.
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When the medium is cool, plug the vials with cotton
batting or rubber foam plugs.
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